Services

Signal Check — free

Send up to 5 Gsamples of BLOW5 signal and, if you have a question, up to three targets. You learn whether your signal decodes as-is, needs a calibration set, or needs an alphabet extension. If it decodes, you receive the full result set on that subset — sequence, modifications, reconstruction, audit record, viewer — and the target calls. One Signal Check per institution and per pore/chemistry. Non-human data: nothing to sign, just the request form. Human-derived research data: after an AVV is in place.

Start a Signal Check

Specified Search Run — “is it in it?”

You specify what you are looking for: sequences, constructs, loci on a reference, or modification positions. You set the thresholds. We search your raw signal for your targets and for an automatically generated decoy set, without basecalling the run, and report per target: evidence found, not observed at this depth, or inconclusive — with supporting reads, identity, coverage breadth and depth, the decoy hit rate, the evidence reads themselves, and the reconstruction of the top evidence reads. Only the evidence reads are basecalled, so you can inspect them; full FASTQ/BAM of the whole run is an add-on.

From €249 per run — from €11 per barcode when multiplexed. Repeat searches on a retained job from €99.

This search is for research use only. No clinical, diagnostic, forensic, or regulatory decision depends on its result.

Patient specimens and sequences that serve identification of a natural person are not accepted.

How it works and what you can search for

Decode Run

Your signal in; FASTQ, BAM with modification tags (5mC, 5hmC, 6mA, 4mC) — unaligned unless you give us a reference — VCF/PAF when you give us a reference, the .sen-lite reconstruction of a representative sample of reads and of every low-confidence read, per-read QC, the signed audit record and the viewer out. Full-run .sen-lite on request. Priced by size, known before anything runs.

From €349 per run — from €15 per barcode when multiplexed.

Pricing

Calibration — one-time, per pore and chemistry

When your signal is new to us. Step one is feasibility: we run a learning curve on the labelled reads you already have and send you a written spec of what, if anything, is missing — you can stop there. Step two is the calibration itself: we adapt the decoder's front end on a few thousand of your labelled reads; the core stays frozen and hash-asserted. You receive the learning-curve report, a decode of your data, and a calibration ID that every later Decode Run or Search Run on that pore and chemistry reuses at list price.

Feasibility €900, credited to the calibration. Calibration €4,900.

If you cannot produce the calibration set yourself, we can sequence your control material in our lab as part of the calibration — see Lab sequencing for calibration.

The same track builds a reference genome when your organism has none. A Specified Search Run needs something to align to, and an assembly built on the fly would give you coordinates nobody can reproduce — so it starts where a Calibration starts, with a feasibility stage that ends in a written answer about whether your data supports an assembly at all.

When there is no model Lab sequencing for calibration

Alphabet Extension — project

A new base, a new analyte, a new alphabet. We derive codebook rows from a molecular structure (CCD code, PubChem CID, MOL2 or SMILES) or from a level table you supply, adapt the decoder on your calibration data, decode your reads, and deliver a report and the viewer. Afterwards, a Specified Search Run can look for your extended symbols. Three milestones, each contracted and delivered on its own: codebook rows, calibration and adaptation, decode and report.

From €9,900.

The Alphabet Extension project